| First Author | Humbert PO | Year | 1997 |
| Journal | J Immunol | Volume | 159 |
| Issue | 11 | Pages | 5273-84 |
| PubMed ID | 9548466 | Mgi Jnum | J:44273 |
| Mgi Id | MGI:1099659 | Doi | 10.4049/jimmunol.159.11.5273 |
| Citation | Humbert PO, et al. (1997) oct-2 gene disruption eliminates the peritoneal B-1 lymphocyte lineage and attenuates B-2 cell maturation and function. J Immunol 159(11):5273-84 |
| abstractText | Targeted mutation of the gene for the Oct-2 transcription factor in mice caused neonatal lethality and abrogated mitogen-induced proliferation and differentiation of mature B lymphocytes in vitro. Here we show that Oct-2 is required for normal humoral responses upon immunization with T cell-dependent as well as T-independent Ags. oct-2-null T cell behavior was normal, implying a B cell-restricted lesion. oct-2-/- B cells displayed aberrant behavior during activation in vitro: both acquisition of markers of cellular activation and cell survival were diminished. Production of early B lineage cells in the bone marrow was normal, yet mature B cells were under-represented in blood and lymphoid organs. Furthermore, peritoneal B-1 lymphocytes were not detected in animals with a reconstituted oct-2-/- lymphoid system. We conclude that Oct-2 is required for B-1 cell maintenance and for normal Ag-driven maturation of conventional B cells in vivo. |
