| Primary Identifier | MGI:3033707 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd276 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Homologous recombination was used to replace a 3.2 kb genomic fragment including the exon encoding the second immunoglobulin domain with a neomycin cassette in antisense orientation relative to transcription. RT-PCR and flow cytometric analysis indicated an absence of transcript and protein, respectively, in homozygous mutant MEFs. |
