| Primary Identifier | MGI:3037977 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ebf2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing tau-GFPpA reporter with a floxed tk-neo component was inserted in the place of exons 1-5 by homologous recombination, and remained under transcriptional control of the endogenous promoter. The exons encoded the first 162 amino acids, which contain domains crucial for DNA binding and transactivation of the protein binding site. Subsequent cre-mediated recombination removed the tk-neo cassette. In situ hybridization indicated lack of endogenous gene expression. The expression pattern of GFP recapitulated that of the endogenous gene. |
