| First Author | Sun Y | Year | 2013 |
| Journal | Carcinogenesis | Volume | 34 |
| Issue | 3 | Pages | 595-604 |
| PubMed ID | 23188673 | Mgi Jnum | J:194680 |
| Mgi Id | MGI:5474511 | Doi | 10.1093/carcin/bgs372 |
| Citation | Sun Y, et al. (2013) Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma. Carcinogenesis 34(3):595-604 |
| abstractText | To date, the useful markers of hepatocellular carcinoma (HCC) remains incompletely developed. Here, we show that annexin A2 complement alpha-fetoprotein (AFP), a widely used liver cancer marker, in the serologically surveillance and early detection of HCC. First, differentially expressed proteins in HCC were identified using a subcellular proteomic approach. Annexin A2 was then selected for further verification. It was found to be overexpressed in HCC tissues (60.7%, 136/224). Using a self-established sandwich enzyme-linked immunosorbent assay, we found that annexin A2 significantly increased in the sera of HCC (n = 175, median, 24.75 ng/microl) compared with the healthy (n = 49, median, 16.69 ng/microl), benign tumors (n = 19, median, 19.92 ng/microl), hepatitis (n = 23, median, 6.48 ng/microl) and cirrhosis (n = 51, median, 7.39 ng/microl) controls and other malignant tumors (n = 87). Importantly, raised concentrations of annexin A2 were observed in 83.2% (79/95) of early stage (median, 24.32 ng/microl) and 78.4% (58/74) of AFP-negative (median, 24.09 ng/microl) patients. Annexin A2 alone had a better area under the receiver-operating characteristic curve (AUC = 0.79, 95% confidence interval: 0.73-0.85) in comparison with AFP (AUC = 0.73, 95% confidence interval: 0.66-0.80) in detecting of early stage HCC. Combining both markers notably improved the diagnostic efficiency of early HCC with an achieved sensitivity of 87.4%. Additionally, the expression characteristics of annexin A2 during hepatocarcinogenesis were detected in p21-HBx gene knockin transgenic mice model. The results showed that annexin A2 expression was substantially elevated in HCC-bearing mice, in accordance with the finding in human samples. In conclusion, annexin A2 may be an independent serological candidate for hepatitis B virus-related HCC, especially in the early stage cases with normal serum AFP. |
