| First Author | Qu J | Year | 2019 |
| Journal | J Immunol Res | Volume | 2019 |
| Pages | 2691808 | PubMed ID | 31930147 |
| Mgi Jnum | J:292422 | Mgi Id | MGI:6448855 |
| Doi | 10.1155/2019/2691808 | Citation | Qu J, et al. (2019) TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of Schistosoma japonicum-Infected C57BL/6 Mice. J Immunol Res 2019:2691808 |
| abstractText | Toll-like receptors (TLRs) play an important role in regulating immune responses during pathogen infection. However, roles of TLRs on T cells reside in the mesenteric lymph node (MLN) were not be fully elucidated in the course of S. japonicum infection. In this study, T lymphocytes from the mesenteric lymph node (MLN) of S. japonicum-infected mice were isolated and the expression and roles of TLR2, TLR3, TLR4, and TLR7 on both CD4(+) and CD8(+) T cells were compared. We found that the expression of TLR7 was increased in the MLN cells of S. japonicum-infected mice, particularly in CD4(+) and CD8(+) T cells (P < 0.05). R848, a TLR7 agonist, could enhance the production of IFN-gamma from MLN T cells of infected mice (P < 0.05), especially in CD8(+) T cells (P < 0.01). In TLR7 gene knockedout (KO) mice, the S. japonicum infection caused a significant decrease (P < 0.05) of the expression of CD25 and CD69, as well as the production of IFN-gamma and IL-4 inducted by PMA plus ionomycin on both CD4(+) and CD8(+) T cells. Furthermore, the decreased level of IFN-gamma and IL-4 in the supernatants of SEA- or SWA-stimulated mesenteric lymphocytes was detected (P < 0.05). Our results indicated that S. japonicum infection could induce the TLR7 expression on T cells in the MLN of C57BL/6 mice, and TLR7 mediates T cell response in the early phase of infection. |
