| First Author | Takahashi K | Year | 2002 |
| Journal | Microbes Infect | Volume | 4 |
| Issue | 8 | Pages | 773-84 |
| PubMed ID | 12270724 | Mgi Jnum | J:90437 |
| Mgi Id | MGI:3043864 | Doi | 10.1016/s1286-4579(02)01597-6 |
| Citation | Takahashi K, et al. (2002) Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis. Microbes Infect 4(8):773-84 |
| abstractText | The mannose-binding lectin (MBL) (also known as the mannose-binding protein) is a serum protein that plays a role as an 'ante-antibody' in innate immunity. In man, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, MBL-A and MBL-C. In order to evaluate the relative roles of these two forms of MBL, we created MBL-A null mice that were MBL-C sufficient. We found MBL-A null mice had enhanced survival in a septic peritonitis model compared to wild-type mice and complement 3 null mice at 24 h, 48 h and 10 d (P < 0.05). Reconstitution of these mice with human MBL reversed the phenotype. Surviving mice had significantly decreased TNF-alpha and IL-6 levels in the blood and peritoneal cavity (P < 0.01). In vitro studies indicate that bacteria opsonized with MBL-A-deficient serum induced significantly less cytokine by peritoneal macrophages compared to those with wild-type serum. Our results indicate that MBL-A is a modulator of inflammation in vivo and in vitro in the mouse and that the role of MBL may extend beyond its role as an opsonin. |
