| Primary Identifier | MGI:3043637 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Fem1c |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous gene was disrupted by the insertion of the ROSA vector containing a beta-galactosidase/neo fusion protein into intron 1. While sequence analysis of RT-PCR products showed splicing between exon 1 and the ROSA cassette and did not detect transcript in which exon 1 was spliced to exon 2, transcript containing exons 2 and 3 (encompassing the entire coding region) was detected in some tissues, including testes and brain, but this latter transcript was not detected in eye, kidney, or cardiac muscle tissue. |
