| First Author | McCracken LM | Year | 2017 |
| Journal | Proc Natl Acad Sci U S A | Volume | 114 |
| Issue | 34 | Pages | E7179-E7186 |
| PubMed ID | 28784756 | Mgi Jnum | J:244005 |
| Mgi Id | MGI:5912784 | Doi | 10.1073/pnas.1703839114 |
| Citation | McCracken LM, et al. (2017) Glycine receptor alpha3 and alpha2 subunits mediate tonic and exogenous agonist-induced currents in forebrain. Proc Natl Acad Sci U S A 114(34):E7179-E7186 |
| abstractText | Neuronal inhibition can occur via synaptic mechanisms or through tonic activation of extrasynaptic receptors. In spinal cord, glycine mediates synaptic inhibition through the activation of heteromeric glycine receptors (GlyRs) composed primarily of alpha1 and beta subunits. Inhibitory GlyRs are also found throughout the brain, where GlyR alpha2 and alpha3 subunit expression exceeds that of alpha1, particularly in forebrain structures, and coassembly of these alpha subunits with the beta subunit appears to occur to a lesser extent than in spinal cord. Here, we analyzed GlyR currents in several regions of the adolescent mouse forebrain (striatum, prefrontal cortex, hippocampus, amygdala, and bed nucleus of the stria terminalis). Our results show ubiquitous expression of GlyRs that mediate large-amplitude currents in response to exogenously applied glycine in these forebrain structures. Additionally, tonic inward currents were also detected, but only in the striatum, hippocampus, and prefrontal cortex (PFC). These tonic currents were sensitive to both strychnine and picrotoxin, indicating that they are mediated by extrasynaptic homomeric GlyRs. Recordings from mice deficient in the GlyR alpha3 subunit (Glra3-/-) revealed a lack of tonic GlyR currents in the striatum and the PFC. In Glra2-/Y animals, GlyR tonic currents were preserved; however, the amplitudes of current responses to exogenous glycine were significantly reduced. We conclude that functional alpha2 and alpha3 GlyRs are present in various regions of the forebrain and that alpha3 GlyRs specifically participate in tonic inhibition in the striatum and PFC. Our findings suggest roles for glycine in regulating neuronal excitability in the forebrain. |
