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Publication : Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells.

First Author  Luo X Year  2024
Journal  BMC Biol Volume  22
Issue  1 Pages  176
PubMed ID  39183304 Mgi Jnum  J:353442
Mgi Id  MGI:7713512 Doi  10.1186/s12915-024-01957-3
Citation  Luo X, et al. (2024) Casein kinase 1alpha mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells. BMC Biol 22(1):176
abstractText  BACKGROUND: Casein kinase 1alpha (CK1alpha), expressed in both ovarian germ and somatic cells, is involved in the initial meiosis and primordial follicle formation of mouse oocytes. Using in vitro and in vivo experiments in this study, we explored the function and mechanism of CK1alpha in estrogen synthesis in mice ovarian granulosa cells. METHODS: A CK1alpha knockout (cKO) mouse model, targeted specifically to ovarian granulosa cells (GCs), was employed to establish the influence of CK1alpha on in vivo estrogen synthesis. The influence of CK1alpha deficiency on GCs was determined in vivo and in vitro by immunofluorescence analysis and Western blot assay. Transcriptome profiling, differentially expressed genes and gene functional enrichment analyses, and computation protein-protein docking, were further employed to assess the CK1alpha pathway. Furthermore, wild-type female mice were treated with the CK1alpha antagonist D4476 to elucidate the CK1alpha's role in estrogen regulation. RESULTS: Ovarian GCs CK1alpha deficiency impaired fertility and superovulation of female mice; also, the average litter size and the estradiol (E(2)) level in the serum of cKO female mice were decreased by 57.3% and 87.4% vs. control mice, respectively. This deficiency disrupted the estrous cycle and enhanced the apoptosis in the GCs. We observed that CK1alpha mediated the secretion of estradiol in mouse ovarian GCs via the cytochrome P450 subfamily 19 member 1 (CYP19A1). CONCLUSIONS: These findings improve the existing understanding of the regulation mechanism of female reproduction and estrogen synthesis. TRIAL REGISTRATION: Not applicable.
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