| First Author | Fujimoto K | Year | 2011 |
| Journal | J Immunol | Volume | 186 |
| Issue | 11 | Pages | 6287-95 |
| PubMed ID | 21525388 | Mgi Jnum | J:173183 |
| Mgi Id | MGI:5013515 | Doi | 10.4049/jimmunol.1004036 |
| Citation | Fujimoto K, et al. (2011) A New Subset of CD103+CD8{alpha}+ Dendritic Cells in the Small Intestine Expresses TLR3, TLR7, and TLR9 and Induces Th1 Response and CTL Activity. J Immunol 186(11):6287-95 |
| abstractText | CD103(+) dendritic cells (DCs) are the major conventional DC population in the intestinal lamina propria (LP). Our previous report showed that a small number of cells in the LP could be classified into four subsets based on the difference in CD11c/CD11b expression patterns: CD11c(hi)CD11b(lo) DCs, CD11c(hi)CD11b(hi) DCs, CD11c(int)CD11b(int) macrophages, and CD11c(int)CD11b(hi) eosinophils. The CD11c(hi)CD11b(hi) DCs, which are CD103(+), specifically express TLR5 and induce the differentiation of naive B cells into IgA(+) plasma cells. These DCs also mediate the differentiation of Ag-specific Th17 and Th1 cells in response to flagellin. We found that small intestine CD103(+) DCs of the LP (LPDCs) could be divided into a small subset of CD8alpha(+) cells and a larger subset of CD8alpha(-) cells. Flow cytometry analysis revealed that CD103(+)CD8alpha(+) and CD103(+)CD8alpha(-) LPDCs were equivalent to CD11c(hi)CD11b(lo) and CD11c(hi)CD11b(hi) subsets, respectively. We analyzed a novel subset of CD8alpha(+) LPDCs to elucidate their immunological function. CD103(+)CD8alpha(+) LPDCs expressed TLR3, TLR7, and TLR9 and produced IL-6 and IL-12p40, but not TNF-alpha, IL-10, or IL-23, following TLR ligand stimulation. CD103(+)CD8alpha(+) LPDCs did not express the gene encoding retinoic acid-converting enzyme Raldh2 and were not involved in T cell-independent IgA synthesis or Foxp3(+) regulatory T cell induction. Furthermore, CD103(+)CD8alpha(+) LPDCs induced Ag-specific IgG in serum, a Th1 response, and CTL activity in vivo. Accordingly, CD103(+)CD8alpha(+) LPDCs exhibit a different function from CD103(+)CD8alpha(-) LPDCs in active immunity. This is the first analysis, to our knowledge, of CD8alpha(+) DCs in the LP of the small intestine. |
