| First Author | Dong B | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 11 | Pages | e80500 |
| PubMed ID | 24260404 | Mgi Jnum | J:225176 |
| Mgi Id | MGI:5691659 | Doi | 10.1371/journal.pone.0080500 |
| Citation | Dong B, et al. (2013) Mammalian diaphanous-related formin 1 regulates GSK3beta-dependent microtubule dynamics required for T cell migratory polarization. PLoS One 8(11):e80500 |
| abstractText | The mammalian diaphanous-related formin (mDia1), a Rho-regulated cytoskeletal modulator, has been shown to promote T lymphocyte chemotaxis and interaction with antigen presenting cells, but the mechanisms underpinning mDia1 roles in these processes have not been defined. Here we show that mDia1(-/-) T cells exhibit impaired lymphocyte function-associated antigen 1 (LFA-1)-mediated T cell adhesion, migration and in vivo trafficking. These defects are associated with impaired microtubule (MT) polarization and stabilization, altered MT dynamics and reduced peripheral clustering of the MT plus-end-protein, adenomatous polyposis coli (APC) in migrating T cells following LFA-1-engagement. Loss of mDia1 also leads to impaired inducible inactivation of the glycogen synthase kinase (GSK) 3beta as well as hyperphosphorylation and reduced levels of APC in migrating T cells. These findings identify essential roles for the mDia1 formin in modulating GSK3beta-dependent MT contributions to induction of T-cell polarity, adhesion and motility. |
