| First Author | Li M | Year | 2001 |
| Journal | J Immunol | Volume | 166 |
| Issue | 10 | Pages | 6099-103 |
| PubMed ID | 11342628 | Mgi Jnum | J:107361 |
| Mgi Id | MGI:3620894 | Doi | 10.4049/jimmunol.166.10.6099 |
| Citation | Li M, et al. (2001) Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo. J Immunol 166(10):6099-103 |
| abstractText | To better understand the antigenic requirements for cross-presentation, we compared the in vivo efficiency of presentation of cell-associated vs soluble OVA with the OT-I (CD8) and OT-II (CD4) TCR transgenic lines. Cross-presentation of cell-associated OVA was very efficient, requiring as little as 21 ng of OVA to activate OT-II cells and 100-fold less to activate OT-I cells. In contrast, soluble OVA was presented inefficiently, requiring at least 10,000 ng OVA for activation of either T cell subset. Thus, cell-associated OVA was presented 500-fold more efficiently than soluble OVA to CD4 T cells and 50,000-fold more efficiently to CD8 T cells. These data, which represent the first quantitative in vivo analysis of cross-presentation, show that cell-associated OVA is very efficiently presented via the class I pathway. |
