| First Author | Pourmorady AD | Year | 2024 |
| Journal | Nature | Volume | 625 |
| Issue | 7993 | Pages | 181-188 |
| PubMed ID | 38123679 | Mgi Jnum | J:357383 |
| Mgi Id | MGI:7645559 | Doi | 10.1038/s41586-023-06845-4 |
| Citation | Pourmorady AD, et al. (2024) RNA-mediated symmetry breaking enables singular olfactory receptor choice. Nature 625(7993):181-188 |
| abstractText | Olfactory receptor (OR) choice provides an extreme example of allelic competition for transcriptional dominance, where every olfactory neuron stably transcribes one of approximately 2,000 or more OR alleles(1,2). OR gene choice is mediated by a multichromosomal enhancer hub that activates transcription at a single OR(3,4), followed by OR-translation-dependent feedback that stabilizes this choice(5,6). Here, using single-cell genomics, we show formation of many competing hubs with variable enhancer composition, only one of which retains euchromatic features and transcriptional competence. Furthermore, we provide evidence that OR transcription recruits enhancers and reinforces enhancer hub activity locally, whereas OR RNA inhibits transcription of competing ORs over distance, promoting transition to transcriptional singularity. Whereas OR transcription is sufficient to break the symmetry between equipotent enhancer hubs, OR translation stabilizes transcription at the prevailing hub, indicating that there may be sequential non-coding and coding mechanisms that are implemented by OR alleles for transcriptional prevalence. We propose that coding OR mRNAs possess non-coding functions that influence nuclear architecture, enhance their own transcription and inhibit transcription from their competitors, with generalizable implications for probabilistic cell fate decisions. |
