Primary Identifier | MGI:3046998 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Hyou1 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A PGK-neo cassette replaced exons 8-10 via homologous recombination. Southern blot confirmed successful recombination. Immunoblotting of heterozygous mice indicated presence of the full-length protein only and not the predicted 226 amino acid long truncated protein, indicating that the mutation results in a null allele. |