Primary Identifier | MGI:3047686 | Allele Type | Gene trapped |
Attribute String | Reporter | Gene | Rab3ip |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A gene trap vector pPAT containing intron 1 and the splicing acceptor from Fyn, lacZ-pA, Ampr, PGK-neo and the splicing donor from Fyn was inserted at the 3' end of the endogenous gene. Northern blot of mutant mice indicated no wild-type transcripts present in brain, testis or kidney, but showed evidence of fusion transcripts between the neo and the 3' end of Rab3ip. RT-PCR of mutant kidney and testis confirmed lack of wild-type transcript. |