| First Author | Rodda LB | Year | 2018 |
| Journal | Immunity | Volume | 48 |
| Issue | 5 | Pages | 1014-1028.e6 |
| PubMed ID | 29752062 | Mgi Jnum | J:268924 |
| Mgi Id | MGI:6269054 | Doi | 10.1016/j.immuni.2018.04.006 |
| Citation | Rodda LB, et al. (2018) Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity. Immunity 48(5):1014-1028.e6 |
| abstractText | Stromal cells (SCs) establish the compartmentalization of lymphoid tissues critical to the immune response. However, the full diversity of lymph node (LN) SCs remains undefined. Using droplet-based single-cell RNA sequencing, we identified nine peripheral LN non-endothelial SC clusters. Included are the established subsets, Ccl19(hi) T-zone reticular cells (TRCs), marginal reticular cells, follicular dendritic cells (FDCs), and perivascular cells. We also identified Ccl19(lo) TRCs, likely including cholesterol-25-hydroxylase(+) cells located at the T-zone perimeter, Cxcl9(+) TRCs in the T-zone and interfollicular region, CD34(+) SCs in the capsule and medullary vessel adventitia, indolethylamine N-methyltransferase(+) SCs in the medullary cords, and Nr4a1(+) SCs in several niches. These data help define how transcriptionally distinct LN SCs support niche-restricted immune functions and provide evidence that many SCs are in an activated state. |
