| First Author | Cacheux M | Year | 2019 |
| Journal | Circ Arrhythm Electrophysiol | Volume | 12 |
| Issue | 11 | Pages | e007382 |
| PubMed ID | 31726860 | Mgi Jnum | J:293117 |
| Mgi Id | MGI:6451318 | Doi | 10.1161/CIRCEP.119.007382 |
| Citation | Cacheux M, et al. (2019) Cardiomyocyte-Specific STIM1 (Stromal Interaction Molecule 1) Depletion in the Adult Heart Promotes the Development of Arrhythmogenic Discordant Alternans. Circ Arrhythm Electrophysiol 12(11):e007382 |
| abstractText | BACKGROUND: STIM1 (stromal interaction molecule 1) is a calcium (Ca(2+)) sensor that regulates cardiac hypertrophy by triggering store-operated Ca(2+) entry. Because STIM1 binding to phospholamban increases sarcoplasmic reticulum Ca(2+) load independent of store-operated Ca(2+) entry, we hypothesized that it controls electrophysiological function and arrhythmias in the adult heart. METHODS: Inducible myocyte-restricted STIM1-KD (STIM1 knockdown) was achieved in adult mice using an alphaMHC (alpha-myosin heavy chain)-MerCreMer system. Mechanical and electrophysiological properties were examined using echocardiography in vivo and optical action potential (AP) mapping ex vivo in tamoxifen-induced STIM1(flox/flox)-Cre(tg)(/-) (STIM1-KD) and littermate controls for STIM1(flox/flox) (referred to as STIM1-Ctl) and for Cre(tg/-) without STIM deletion (referred to as Cre-Ctl). RESULTS: STIM1-KD mice (N=23) exhibited poor survival compared with STIM1-Ctl (N=22) and Cre-Ctl (N=11) with >50% mortality after only 8-days of cardiomyocyte-restricted STIM1-KD. STIM1-KD but not STIM1-Ctl or Cre-Ctl hearts exhibited a proclivity for arrhythmic behavior, ranging from frequent ectopy to pacing-induced ventricular tachycardia/ventricular fibrillation (VT/VF). Examination of the electrophysiological substrate revealed decreased conduction velocity and increased AP duration (APD) heterogeneity in STIM1-KD. These features, however, were comparable in VT/VF(+) and VT/VF(-) hearts. We also uncovered a marked increase in the magnitude of APD alternans during rapid pacing, and the emergence of a spatially discordant alternans profile in STIM1-KD hearts. Unlike conduction velocity slowing and APD heterogeneity, the magnitude of APD alternans was greater (by 80%, P<0.05) in VT/VF(+) versus VT/VF(-) STIM1-KD hearts. Detailed phase mapping during the initial beats of VT/VF identified one or more rotors that were localized along the nodal line separating out-of-phase alternans regions. CONCLUSIONS: In an adult murine model with inducible and myocyte-specific STIM1 depletion, we demonstrate for the first time the regulation of spatially discordant alternans by STIM1. Early mortality in STIM1-KD mice is likely related to enhanced susceptibility to VT/VF secondary to discordant APD alternans. |
