| Primary Identifier | MGI:3050805 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nfat5 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo was inserted in intron 5 and a loxP site in intron 7. Transient Cre expression deleted the larger floxed region, including exons 6 and 7. These exons encode amino acids 254-380, which comprise the N-terminal portion of the rel DNA-binding domain (DBD). Germ line transmission was verified by PCR and Southern blot. RNA and protein expression in mutant MEFs showed that the allele encodes a truncated protein containing an internal deletion as expected. Immunoreactivity was eliminated as demonstrated by using antisera against the DBD. |
