| Primary Identifier | MGI:3050819 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Lmnb1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A gene trap containing a Beta-geo reporter gene was inserted into intron 5, resulting in a fusion transcript containing the endogenous amino-terminal head domain and a truncated alpha-helical central rod domain fused to Beta-geo. The protein lacked the endogenous carboxyl-terminal 273 amino acids of Lmb1, thereby eliminating one of two known phosphorylation sites, the nuclear localization signal, and the carboxyl-terminal CAAX motif. Only the 5' sequences were detected in mutant embryos by RT-PCR and Western blot showed no protein. |
