| First Author | Borah BJ | Year | 2021 |
| Journal | iScience | Volume | 24 |
| Issue | 9 | Pages | 103041 |
| PubMed ID | 34585109 | Mgi Jnum | J:350605 |
| Mgi Id | MGI:6798057 | Doi | 10.1016/j.isci.2021.103041 |
| Citation | Borah BJ, et al. (2021) Nyquist-exceeding high voxel rate acquisition in mesoscopic multiphoton microscopy for full-field submicron resolution resolvability. iScience 24(9):103041 |
| abstractText | The Nyquist-Shannon criterion has never been realized in a laser-scanning mesoscopic multiphoton microscope (MPM) with a large field-of-view (FOV)-resolution ratio, especially when employing a high-frequency resonant-raster-scanning. With a high optical resolution nature, a current mesoscopic-MPM either neglects the criterion and degrades the digital resolution to twice the pixel size, or reduces the FOV and/or the raster-scanning speed to avoid aliasing. We introduce a Nyquist figure-of-merit (NFOM) parameter to characterize a laser-scanning MPM in terms of its optical-resolution retrieving ability. Based on NFOM, we define the maximum aliasing-free FOV, and subsequently, a cross-over excitation wavelength, below which the FOV becomes NFOM-constrained irrespective of an optimized optical design. We validate our idea in a custom-built mesoscopic-MPM with millimeter-scale FOV yielding an ultra-high FOV-resolution ratio of >3,000, while securing up-to a 1.6 mm Nyquist-satisfied aliasing-free FOV, a approximately 400 nm lateral resolution, and a 70 M/s effective voxel-sampling rate, all at the same time. |
