| First Author | Xu R | Year | 2020 |
| Journal | Biomed Pharmacother | Volume | 130 |
| Pages | 110581 | PubMed ID | 32745914 |
| Mgi Jnum | J:315757 | Mgi Id | MGI:6820863 |
| Doi | 10.1016/j.biopha.2020.110581 | Citation | Xu R, et al. (2020) Radix Rehmanniae Praeparata promotes bone fracture healing through activation of TGF-beta signaling in mesenchymal progenitors. Biomed Pharmacother 130:110581 |
| abstractText | BACKGROUND: Radix Rehmanniae Praeparata (RR), the steamed roots of Rehmannia glutinosa, is a traditional Chinese medicine with the function of kidney-nourishing, and it has been safety used for centuries to treat bone-related disorders. The aim of this study is to investigate the positive effect and underlying mechanism of RR enhancing bone fracture healing in mouse model. METHODS: Ten-week-old C57BL/6J mice were subjected to a unilateral open transverse tibial fracture and provided a daily treatment of RR. Bone samples were harvested for tissue analyses including x-ray, muCT, histology, histomorphometry, biomechanical testing, immunohistochemical (IHC) and quantitative gene expression analysis. To determine the role of TGF-beta in accelerating fracture healing effect of RR, aforementioned experiments were performed on Gli1-CreER; Tgfbr2 f(lox/flox) (Tgfbr2(Gli1ER)) conditional knockout mice. RESULTS: RR promoted bone fracture healing and strengthened bone intensity in wild-type and Cre(-) mice with the activation of TGF-beta/Smad2 signaling, on the contrary, RR failed to accelerating fracture healing in Tgfbr2(Gli1ER) mice. CONCLUSION: RR promotes bone fracture healing by intensify the contribution of Gli1+ cells on bone and cartilage formation mainly in TGF-beta-dependent manner. RR is an alternative option for clinical treatment of fracture. |
