| First Author | Marg A | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 401 |
| Issue | 1 | Pages | 143-8 |
| PubMed ID | 20833135 | Mgi Jnum | J:165850 |
| Mgi Id | MGI:4838689 | Doi | 10.1016/j.bbrc.2010.09.030 |
| Citation | Marg A, et al. (2010) AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness. Biochem Biophys Res Commun 401(1):143-8 |
| abstractText | The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using atomic force microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1/ fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle. |
