| Primary Identifier | MGI:3053973 | Allele Type | Transgenic |
| Attribute String | Inducible, Reporter | Gene | Tg(tetop-lacZ)2Mam |
| Strain of Origin | C57BL/6 x SJL | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| description | Four lines were produced (G1-G4). In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene. |
| molecularNote | The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. |
