| First Author | Miyagawa F | Year | 2013 |
| Journal | J Invest Dermatol | Volume | 133 |
| Issue | 11 | Pages | 2538-2545 |
| PubMed ID | 23648548 | Mgi Jnum | J:202379 |
| Mgi Id | MGI:5518964 | Doi | 10.1038/jid.2013.212 |
| Citation | Miyagawa F, et al. (2013) Identification of CD3(+)CD4(-)CD8(-) T Cells as Potential Regulatory Cells in an Experimental Murine Model of Graft-Versus-Host Skin Disease (GVHD). J Invest Dermatol 133(11):2538-45 |
| abstractText | We have developed K14-mOVA transgenic (Tg) mice that express membrane-associated ovalbumin (mOVA) under the control of a K14 promoter, as well as double Tg mice, by crossing them with OT-I mice that have a TCR recognizing the OVA peptide. When injected with CD8(+) OT-I cells, K14-mOVA Tg mice develop graft-versus-host disease (GVHD), whereas double Tg mice are protected. This suggests that, in double Tg mice, regulatory mechanisms may prevent infused OT-I cells from inducing GVHD. We demonstrated that, after adoptive transfer, TCRalphabeta(+)CD3(+)CD4(-)CD8(-)NK1.1(-) double-negative (DN) T cells are increased in the peripheral lymphoid organs and skin of double Tg mice and exhibit a Valpha2(+)Vbeta5(+)TCR that has the same TCR specificity as OT-I cells. These DN T cells isolated from tolerant double Tg mice proliferated in response to OVA peptide and produced IFN-gamma in the presence of IL-2. These cells could also suppress the proliferation of OT-I cells and were able to specifically kill activated OT-I cells through Fas/Fas ligand interaction. These findings suggest that DN T cells that accumulate in double Tg mice have regulatory functions and may have a role in the maintenance of peripheral tolerance in vivo. |
