| First Author | Cannons JL | Year | 2021 |
| Journal | Cell Rep | Volume | 37 |
| Issue | 2 | Pages | 109804 |
| PubMed ID | 34644563 | Mgi Jnum | J:334660 |
| Mgi Id | MGI:6881812 | Doi | 10.1016/j.celrep.2021.109804 |
| Citation | Cannons JL, et al. (2021) PI3Kdelta coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8(+) T cells at the expense of central memory. Cell Rep 37(2):109804 |
| abstractText | Patients with activated phosphatidylinositol 3-kinase delta (PI3Kdelta) syndrome (APDS) present with sinopulmonary infections, lymphadenopathy, and cytomegalvirus (CMV) and/or Epstein-Barr virus (EBV) viremia, yet why patients fail to clear certain chronic viral infections remains incompletely understood. Using patient samples and a mouse model (Pik3cd(E1020K/+) mice), we demonstrate that, upon activation, Pik3cd(E1020K/+) CD8(+) T cells exhibit exaggerated features of effector populations both in vitro and after viral infection that are associated with increased Fas-mediated apoptosis due to sustained FoxO1 phosphorylation and Fasl derepression, enhanced mTORC1 and c-Myc signatures, metabolic perturbations, and an altered chromatin landscape. Conversely, Pik3cd(E1020K/+) CD8(+) cells fail to sustain expression of proteins critical for central memory, including TCF1. Strikingly, activated Pik3cd(E1020K/+) CD8(+) cells exhibit altered transcriptional and epigenetic circuits characterized by pronounced interleukin-2 (IL-2)/STAT5 signatures and heightened IL-2 responses that prevent differentiation to memory-like cells in IL-15. Our data position PI3Kdelta as integrating multiple signaling nodes that promote CD8(+) T cell effector differentiation, providing insight into phenotypes of patients with APDS. |
