| First Author | Dowling MR | Year | 2014 |
| Journal | Proc Natl Acad Sci U S A | Volume | 111 |
| Issue | 17 | Pages | 6377-82 |
| PubMed ID | 24733943 | Mgi Jnum | J:208836 |
| Mgi Id | MGI:5565098 | Doi | 10.1073/pnas.1322420111 |
| Citation | Dowling MR, et al. (2014) Stretched cell cycle model for proliferating lymphocytes. Proc Natl Acad Sci U S A 111(17):6377-82 |
| abstractText | Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for dividing lymphocytes where all parts of the cell cycle are proportional to total division time. Data fitting based on a stretched cell cycle model can significantly improve estimates of cell cycle parameters drawn from DNA labeling data used to monitor immune cell dynamics. |
