| First Author | Majri SS | Year | 2018 |
| Journal | J Immunol | Volume | 200 |
| Issue | 1 | Pages | 110-118 |
| PubMed ID | 29187589 | Mgi Jnum | J:253341 |
| Mgi Id | MGI:6108022 | Doi | 10.4049/jimmunol.1701133 |
| Citation | Majri SS, et al. (2018) STAT5B: A Differential Regulator of the Life and Death of CD4(+) Effector Memory T Cells. J Immunol 200(1):110-118 |
| abstractText | Understanding the control of Ag restimulation-induced T cell death (RICD), especially in cancer immunotherapy, where highly proliferating T cells will encounter potentially large amounts of tumor Ags, is important now more than ever. It has been known that growth cytokines make T cells susceptible to RICD, but the precise molecular mediators that govern this in T cell subsets is unknown until now. STAT proteins are a family of transcription factors that regulate gene expression programs underlying key immunological processes. In particular, STAT5 is known to favor the generation and survival of memory T cells. In this study, we report an unexpected role for STAT5 signaling in the death of effector memory T (TEM) cells in mice and humans. TEM cell death was prevented with neutralizing anti-IL-2 Ab or STAT5/JAK3 inhibitors, indicating that STAT5 signaling drives RICD in TEM cells. Moreover, we identified a unique patient with a heterozygous missense mutation in the coiled-coil domain of STAT5B that presented with autoimmune lymphoproliferative syndrome-like features. Similar to Stat5b(-/-) mice, this patient exhibited increased CD4(+) TEM cells in the peripheral blood. The mutant STAT5B protein dominantly interfered with STAT5-driven transcriptional activity, leading to global downregulation of STAT5-regulated genes in patient T cells upon IL-2 stimulation. Notably, CD4(+) TEM cells from the patient were strikingly resistant to cell death by in vitro TCR restimulation, a finding that was recapitulated in Stat5b(-/-) mice. Hence, STAT5B is a crucial regulator of RICD in memory T cells in mice and humans. |
