| First Author | Stohwasser R | Year | 1996 |
| Journal | FEBS Lett | Volume | 383 |
| Issue | 1-2 | Pages | 109-13 |
| PubMed ID | 8612775 | Mgi Jnum | J:32091 |
| Mgi Id | MGI:79596 | Doi | 10.1016/0014-5793(96)00110-x |
| Citation | Stohwasser R, et al. (1996) 20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences. FEBS Lett 383(1-2):109-13 |
| abstractText | 20S proteasomes of tissues from LMP7 knock out mice which show reduced MHC class I restricted antigen presentation were analyzed with regard to their subunit composition, peptide hydrolyzing activity and their ability to cleave a synthetic 25-mer polypeptide. LMP7 deficiency results in an enhanced incorporation of subunit MB1 and in a 2-3.8-fold increase in Vmax for the Suc-LLVY-MCA hydrolyzing activity. Since LMP7 deficiency also affects the cleavage site preference of 20S proteasomes the reduced MHC class I antigen presentation of LMP7 knock out mice is most likely due to an impairment in peptide generation. |
