| First Author | Wang B | Year | 2019 |
| Journal | Cell Rep | Volume | 27 |
| Issue | 12 | Pages | 3473-3485.e5 |
| PubMed ID | 31216469 | Mgi Jnum | J:300614 |
| Mgi Id | MGI:6488863 | Doi | 10.1016/j.celrep.2019.05.068 |
| Citation | Wang B, et al. (2019) Induction of Pluripotent Stem Cells from Mouse Embryonic Fibroblasts by Jdp2-Jhdm1b-Mkk6-Glis1-Nanog-Essrb-Sall4. Cell Rep 27(12):3473-3485.e5 |
| abstractText | Reprogramming somatic cells to pluripotency by Oct4, Sox2, Klf4, and Myc represent a paradigm for cell fate determination. Here, we report a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb, and Sall4 (7F) that reprogram mouse embryonic fibroblasts or MEFs to chimera competent induced pluripotent stem cells (iPSCs) efficiently. RNA sequencing (RNA-seq) and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results establish an alternative paradigm for reprogramming that may be useful for analyzing cell fate control. |
