| First Author | Kumamoto Y | Year | 2016 |
| Journal | Elife | Volume | 5 |
| PubMed ID | 27657168 | Mgi Jnum | J:238018 |
| Mgi Id | MGI:5817860 | Doi | 10.7554/eLife.17979 |
| Citation | Kumamoto Y, et al. (2016) CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens. Elife 5:e17979 |
| abstractText | Strong antibody response is considered a hallmark of a successful vaccine. While dendritic cells (DCs) are important for T follicular helper (Tfh) cell priming, how this process is regulated in vivo is unclear. We show here that the depletion of CD301b+ DCs specifically enhanced the development of Tfh cells, germinal center B cells and antibody responses against protein antigens. Exaggerated antibody responses in mice depleted of CD301b+ DCs occurred in the absence of any adjuvants, and resulting antibodies had broader specificity and higher affinity to the immunogen. CD301b+ DCs express high levels of PD-1 ligands, PD-L1 and PD-L2. Blocking PD-1 or PD-L1 during priming in wild-type mice partially mimicked the phenotype of CD301b+ DC-depleted animals, suggesting their role in Tfh suppression. Transient depletion of CD301b+ DC results in the generation of autoreactive IgG responses. These results revealed a novel regulatory mechanism and a key role of CD301b+ DCs in blocking autoantibody generation. |
