| First Author | Choi JS | Year | 2023 |
| Journal | Cell Rep | Volume | 42 |
| Issue | 9 | Pages | 113037 |
| PubMed ID | 37624694 | Mgi Jnum | J:340669 |
| Mgi Id | MGI:7528572 | Doi | 10.1016/j.celrep.2023.113037 |
| Citation | Choi JS, et al. (2023) Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly. Cell Rep 42(9):113037 |
| abstractText | The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering the mouse SC a popular model to study establishment of neural connectivity. Here we perform single-nucleus RNA-sequencing analysis of the mouse SC isolated at various developmental time points. Our study provides a transcriptomic landscape of the cell types that comprise the SC across murine development with particular emphasis on neuronal heterogeneity. We report a repertoire of genes differentially expressed across the different postnatal ages, many of which are known to regulate axon guidance and synapse formation. Using these data, we find that Pax7 expression is restricted to a subset of GABAergic neurons. Our data provide a valuable resource for interrogating the mechanisms of circuit development and identifying markers for manipulating specific SC neuronal populations and circuits. |
