| Primary Identifier | MGI:3057313 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ereg |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to introduce a nuclear localization signal-tagged lacZ, SV40pA and neo gene into exon 1, immediately upstream of the ATG. PCR revealed a transcript from the PGK promoter, however, Western blot detected a frameshift mutation that rendered the protein nonfunctional. No wild-type transcript or protein was observed in RT-PCR or Western blot of mutants. |
