| First Author | Johnson GA | Year | 2023 |
| Journal | Proc Natl Acad Sci U S A | Volume | 120 |
| Issue | 17 | Pages | e2218617120 |
| PubMed ID | 37068254 | Mgi Jnum | J:354095 |
| Mgi Id | MGI:7542904 | Doi | 10.1073/pnas.2218617120 |
| Citation | Johnson GA, et al. (2023) Merged magnetic resonance and light sheet microscopy of the whole mouse brain. Proc Natl Acad Sci U S A 120(17):e2218617120 |
| abstractText | We have developed workflows to align 3D magnetic resonance histology (MRH) of the mouse brain with light sheet microscopy (LSM) and 3D delineations of the same specimen. We start with MRH of the brain in the skull with gradient echo and diffusion tensor imaging (DTI) at 15 mum isotropic resolution which is ~ 1,000 times higher than that of most preclinical MRI. Connectomes are generated with superresolution tract density images of ~5 mum. Brains are cleared, stained for selected proteins, and imaged by LSM at 1.8 mum/pixel. LSM data are registered into the reference MRH space with labels derived from the ABA common coordinate framework. The result is a high-dimensional integrated volume with registration (HiDiver) with alignment precision better than 50 microm. Throughput is sufficiently high that HiDiver is being used in quantitative studies of the impact of gene variants and aging on mouse brain cytoarchitecture and connectomics. |
