| First Author | Eles JR | Year | 2018 |
| Journal | Biomaterials | Volume | 174 |
| Pages | 79-94 | PubMed ID | 29783119 |
| Mgi Jnum | J:269130 | Mgi Id | MGI:6269141 |
| Doi | 10.1016/j.biomaterials.2018.04.043 | Citation | Eles JR, et al. (2018) In vivo imaging of neuronal calcium during electrode implantation: Spatial and temporal mapping of damage and recovery. Biomaterials 174:79-94 |
| abstractText | Implantable electrode devices enable long-term electrophysiological recordings for brain-machine interfaces and basic neuroscience research. Implantation of these devices, however, leads to neuronal damage and progressive neural degeneration that can lead to device failure. The present study uses in vivo two-photon microscopy to study the calcium activity and morphology of neurons before, during, and one month after electrode implantation to determine how implantation trauma injures neurons. We show that implantation leads to prolonged, elevated calcium levels in neurons within 150mum of the electrode interface. These neurons show signs of mechanical distortion and mechanoporation after implantation, suggesting that calcium influx is related to mechanical trauma. Further, calcium-laden neurites develop signs of axonal injury at 1-3h post-insert. Over the first month after implantation, physiological neuronal calcium activity increases, suggesting that neurons may be recovering. By defining the mechanisms of neuron damage after electrode implantation, our results suggest new directions for therapies to improve electrode longevity. |
