| First Author | Brazão TF | Year | 2016 |
| Journal | Blood | Volume | 128 |
| Issue | 7 | Pages | e10-9 |
| PubMed ID | 27381906 | Mgi Jnum | J:356456 |
| Mgi Id | MGI:7762548 | Doi | 10.1182/blood-2015-11-680843 |
| Citation | Brazao TF, et al. (2016) Long noncoding RNAs in B-cell development and activation. Blood 128(7):e10-9 |
| abstractText | Long noncoding RNAs (lncRNAs) are potentially important regulators of cell differentiation and development, but little is known about their roles in B lymphocytes. Using RNA-seq and de novo transcript assembly, we identified 4516 lncRNAs expressed in 11 stages of B-cell development and activation. Most of these lncRNAs have not been previously detected, even in the closely related T-cell lineage. Comparison with lncRNAs previously described in human B cells identified 185 mouse lncRNAs that have human orthologs. Using chromatin immunoprecipitation-seq, we classified 20% of the lncRNAs as either enhancer-associated (eRNA) or promoter-associated RNAs. We identified 126 eRNAs whose expression closely correlated with the nearest coding gene, thereby indicating the likely location of numerous enhancers active in the B-cell lineage. Furthermore, using this catalog of newly discovered lncRNAs, we show that PAX5, a transcription factor required to specify the B-cell lineage, bound to and regulated the expression of 109 lncRNAs in pro-B and mature B cells and 184 lncRNAs in acute lymphoblastic leukemia. |
