| First Author | Reimer D | Year | 2020 |
| Journal | Cell Rep | Volume | 32 |
| Issue | 6 | Pages | 108030 |
| PubMed ID | 32783949 | Mgi Jnum | J:314419 |
| Mgi Id | MGI:6715872 | Doi | 10.1016/j.celrep.2020.108030 |
| Citation | Reimer D, et al. (2020) B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma Cell Output via Swiprosin-1/EFhd2. Cell Rep 32(6):108030 |
| abstractText | Plasma cells secreting affinity-matured antibodies develop in germinal centers (GCs), where B cells migrate persistently and directionally over defined periods of time. How modes of GC B cell migration influence plasma cell development remained unclear. Through genetic deletion of the F-actin bundling protein Swiprosin-1/EF-hand domain family member 2 (EFhd2) and by two-photon microscopy, we show that EFhd2 restrains B cell speed in GCs and hapten-specific plasma cell output. Modeling the GC reaction reveals that increasing GC B cell speed promotes plasma cell generation. Lack of EFhd2 also reduces contacts of GC B cells with follicular dendritic cells in vivo. Computational modeling uncovers that both GC output and antibody affinity depend quantitatively on contacts of GC B cells with follicular dendritic cells when B cells migrate more persistently. Collectively, our data explain how GC B cells integrate speed and persistence of cell migration with B cell receptor affinity. |
