Primary Identifier | MGI:3510673 | Allele Type | Targeted |
Attribute String | Humanized sequence, Inserted expressed sequence, Reporter | Gene | Ly6a |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert human RUNX1 (CBFA2T1), IRES-EGFP and floxed PGK-neo into exon 2 of the endogenous gene. Expression of the inserted mRNA was detectable in peripheral blood leukocytes from heterozygous animals by RT-PCR. Flow cytometry indicated GFP was highly expressed in peripheral blood and bone marrow cells. |