| Primary Identifier | MGI:3510988 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(KRT5-Erbb2)1Jdg |
| Strain of Origin | FVB | Is Recombinase | false |
| Is Wild Type | false |
| description | Three transgenic founder lines were established. Transgenic line BK5.erbB2A displayed the highest level of transgene expression and was used for all experiments. By 6 months of age, 10% of hemizygous BK5.erbB2A animals and 90% of homozygous BK5.erbB2A animals developed spontaneous papillomas. |
| molecularNote | A rat Erbb2 cDNA under regulatory control of the bovine keratin 5 promoter was used for the transgene. A rabbit beta-globin intron and SV40 polyadenylation sequence was also included in the construct. Transgene expression in basal epidermal cells was confirmed by immunofluorescence of dorsal skin and skin tumor sections. |
