| Primary Identifier | MGI:3513195 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prg4 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct was designed to create a Prg4-B-gal fusion protein by substituting lacZ for exon 6. Northern blot and in situ hybridization, however, detected no transcript in livers of mutant mice. RT-PCR of synovial and liver mRNA showed aberrant mRNA splicing from the allele which was thought to create a nonsense mediated mRNA decay. |
