| Primary Identifier | MGI:3521852 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mmp13 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a PGK-neo and a stop codon in order to splice out exon 5, which encodes the zinc-binding residues in the catalytic domain. Northern blot of mutant calvariae demonstrated a lack of transcript when an exon 5 probe was used and a barely detectable amount of transcript when using a 5' probe. Western blot showed no protein in cultures of mutant calvariae. |
