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Publication : Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin.

First Author  Dey S Year  2024
Journal  STAR Protoc Volume  5
Issue  4 Pages  103312
PubMed ID  39298322 Mgi Jnum  J:359551
Mgi Id  MGI:7787218 Doi  10.1016/j.xpro.2024.103312
Citation  Dey S, et al. (2024) Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin. STAR Protoc 5(4):103312
abstractText  Here, we present a protocol for staining murine skin innervation by either a pan-axonal marker or a genetic tracer of sensory neuron subtypes using floating sections. We also describe steps for using a new MATLAB-based semiautomated routine that facilitates the quantification of innervation density. This protocol can also be applied to other organs, such as the mouse's spinal cord and tongue. For complete details on the use and execution of this protocol, please refer to Dey et al.(1).
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