| Primary Identifier | MGI:3521986 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(11Cops3-Gid4)2Jrl |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A deletion between Cops3 and Gid4 (previously, 4933439F18Rik) was engineered in ES cells by insertion of complementary partial Hprt-loxP constructs at each site. Insertion at Cops3 was specifically targeted whereas insertion in intron 3 of Gid4 was a random insertion brought about using a V15 retrovirus. Cre recombinase actived deleted the intervening region and reconstructed the Hprt minigene for selection purposes. Two additional, phenotypically identical, deletions were created in which the loxP-Hprt partial gene was inserted in intron 5 of Gid4 (MGI:3521943 and MGI:3521944). |
