| Primary Identifier | MGI:3521998 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Ptk2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to introduce loxP sites to flank the exon encoding part of the kinase domain, encoded by amino acids 413-444. Upon transfection with a Cre-expressing plasmid, the floxed PGK-neo cassette was deleted, leaving the exon floxed. The targeting vector was designed so that Cre-mediated recombination would introduce a frameshift was created in the adjacent exon, preventing production of a functional protein. Immunoblot confirmed the lack of wild-type protein or truncated protein created by this allele in Cre-expressing ES cells. |
