| First Author | Horn Z | Year | 2018 |
| Journal | Proc Natl Acad Sci U S A | Volume | 115 |
| Issue | 42 | Pages | 10556-10563 |
| PubMed ID | 30262652 | Mgi Jnum | J:354099 |
| Mgi Id | MGI:6209063 | Doi | 10.1073/pnas.1811100115 |
| Citation | Horn Z, et al. (2018) N-cadherin provides a cis and trans ligand for astrotactin that functions in glial-guided neuronal migration. Proc Natl Acad Sci U S A 115(42):10556-10563 |
| abstractText | Prior studies demonstrate that astrotactin (ASTN1) provides a neuronal receptor for glial-guided CNS migration. Here we report that ASTN1 binds N-cadherin (CDH2) and that the ASTN1:CDH2 interaction supports cell-cell adhesion. To test the function of ASTN1:CDH2 binding in glial-guided neuronal migration, we generated a conditional loss of Cdh2 in cerebellar granule cells and in glia. Granule cell migration was slowed in cerebellar slice cultures after a conditional loss of neuronal Cdh2, and more severe migration defects occurred after a conditional loss of glial Cdh2 Expression in granule cells of a mutant form of ASTN1 that does not bind CDH2 also slowed migration. Moreover, in vitro chimeras of granule cells and glia showed impaired neuron-glia attachment in the absence of glial, but not neuronal, Cdh2 Thus, cis and trans bindings of ASTN1 to neuronal and glial CDH2 form an asymmetric neuron-glial bridge complex that promotes glial-guided neuronal migration. |
