Primary Identifier | MGI:3521830 | Allele Type | Targeted |
Attribute String | Modified isoform(s) | Gene | Met |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to introduce Met exon 14-20 cDNA with M1248T and L1193V mutations downstream of exon 14, changing the ATG and CTT codons to ACG and GTT, respectively. RT-PCR and immunoblotting showed that met RNA and protein expression were equivalent to wild-type. |