| Primary Identifier | MGI:3522422 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Kcnn1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted 40 nucleotides 5' of the initiation methionine codon in exon 3 and a floxed neomycin resistance gene as well as an EGFP gene were inserted in the intron between exons 5 and 6. Crossing to cre-deleter mice led to the excision of the coding portion of exon 3, exon 4 and exon 5 as well as the neo resistance gene. RT-PCR and western blot analysis confirmed the absence of transcription and translation. The EGFP insert was non functional. |
