| First Author | Machová E | Year | 2008 |
| Journal | Neurobiol Aging | Volume | 29 |
| Issue | 3 | Pages | 368-78 |
| PubMed ID | 17140703 | Mgi Jnum | J:135055 |
| Mgi Id | MGI:3790296 | Doi | 10.1016/j.neurobiolaging.2006.10.029 |
| Citation | Machova E, et al. (2008) Impairment of muscarinic transmission in transgenic APPswe/PS1dE9 mice. Neurobiol Aging 29(3):368-78 |
| abstractText | We assessed the integrity of cholinergic neurotransmission in parietal cortex of young adult (7 months) and aged (17 months) transgenic APPswe/PS1dE9 female mice compared to littermate controls. Choline acetyltransferase and acetylcholinesterase activity declined age-dependently in both genotypes, whereas both age- and genotype-dependent decline was found in butyrylcholinesterase activity, vesicular acetylcholine transporter density, muscarinic receptors and carbachol stimulated binding of GTP gamma S in membranes as a functional indicator of muscarinic receptor coupling to G-proteins. Notably, vesicular acetylcholine transporter levels and muscarinic receptor-G-protein coupling were impaired in transgenic mice already at the age of 7 months compared to wild type littermates. Thus, brain amyloid accumulation in this mouse model is accompanied by a serious deterioration of muscarinic transmission already before the mice manifest significant cognitive deficits. |
