| First Author | Masala N | Year | 2024 |
| Journal | Elife | Volume | 13 |
| PubMed ID | 39042440 | Mgi Jnum | J:354919 |
| Mgi Id | MGI:7736872 | Doi | 10.7554/eLife.93804 |
| Citation | Masala N, et al. (2024) Aberrant hippocampal Ca(2+) microwaves following synapsin-dependent adeno-associated viral expression of Ca(2+) indicators. Elife 13 |
| abstractText | Genetically encoded calcium indicators (GECIs) such as GCaMP are invaluable tools in neuroscience to monitor neuronal activity using optical imaging. The viral transduction of GECIs is commonly used to target expression to specific brain regions, can be conveniently used with any mouse strain of interest without the need for prior crossing with a GECI mouse line, and avoids potential hazards due to the chronic expression of GECIs during development. A key requirement for monitoring neuronal activity with an indicator is that the indicator itself minimally affects activity. Here, using common adeno-associated viral (AAV) transduction procedures, we describe spatially confined aberrant Ca(2+) microwaves slowly travelling through the hippocampus following expression of GCaMP6, GCaMP7, or R-CaMP1.07 driven by the synapsin promoter with AAV-dependent gene transfer in a titre-dependent fashion. Ca(2+) microwaves developed in hippocampal CA1 and CA3, but not dentate gyrus nor neocortex, were typically first observed at 4 wk after viral transduction, and persisted up to at least 8 wk. The phenomenon was robust and observed across laboratories with various experimenters and setups. Our results indicate that aberrant hippocampal Ca(2+) microwaves depend on the promoter and viral titre of the GECI, density of expression, as well as the targeted brain region. We used an alternative viral transduction method of GCaMP which avoids this artefact. The results show that commonly used Ca(2+)-indicator AAV transduction procedures can produce artefactual Ca(2+) responses. Our aim is to raise awareness in the field of these artefactual transduction-induced Ca(2+) microwaves, and we provide a potential solution. |
