| First Author | More SS | Year | 2016 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 57 |
| Issue | 7 | Pages | 3231-8 |
| PubMed ID | 27333181 | Mgi Jnum | J:258449 |
| Mgi Id | MGI:6112278 | Doi | 10.1167/iovs.15-17406 |
| Citation | More SS, et al. (2016) Early Detection of Amyloidopathy in Alzheimer's Mice by Hyperspectral Endoscopy. Invest Ophthalmol Vis Sci 57(7):3231-8 |
| abstractText | PURPOSE: To describe a spectral imaging system for small animal studies based on noninvasive endoscopy of the retina, and to present time-resolved spectral changes from live Alzheimer''s mice prior to cognitive decline, corroborating our previous in vitro findings. METHODS: Topical endoscope fundus imaging was modified to use a machine vision camera and tunable wavelength system for acquiring monochromatic images across the visible to near-infrared spectral range. Alzheimer''s APP/PS1 mice and age-matched, wild-type mice were imaged monthly from months 3 through 8 to assess changes in the fundus reflection spectrum. Optical changes were fit to Rayleigh light scatter models as measures of amyloid aggregation. RESULTS: Good quality spectral images of the central retina were obtained. Short-wavelength reflectance from Alzheimer''s mice retinae showed significant reduction over time compared to wild-type mice. Optical changes were consistent with an increase in Rayleigh light scattering in neural retina due to soluble Abeta1-42 aggregates. The changes in light scatter showed a monotonic increase in soluble amyloid aggregates over a 6-month period, with significant build up occurring at 7 months. CONCLUSIONS: Hyperspectral imaging technique can be brought inexpensively to the study of retinal changes caused by Alzheimer''s disease progression in live small animals. A similar previous finding of reduction in the light reflection over a range of wavelengths in isolated Alzheimer''s mice retinae, was reproducible in the living Alzheimer''s mice. The technique presented here has a potential for development as an early Alzheimer''s retinal diagnostic test in humans, which will support the treatment outcome. |
