| First Author | Macauley SL | Year | 2015 |
| Journal | J Clin Invest | Volume | 125 |
| Issue | 6 | Pages | 2463-7 |
| PubMed ID | 25938784 | Mgi Jnum | J:222966 |
| Mgi Id | MGI:5646089 | Doi | 10.1172/JCI79742 |
| Citation | Macauley SL, et al. (2015) Hyperglycemia modulates extracellular amyloid-beta concentrations and neuronal activity in vivo. J Clin Invest 125(6):2463-7 |
| abstractText | Epidemiological studies show that patients with type 2 diabetes (T2DM) and individuals with a diabetes-independent elevation in blood glucose have an increased risk for developing dementia, specifically dementia due to Alzheimer's disease (AD). These observations suggest that abnormal glucose metabolism likely plays a role in some aspects of AD pathogenesis, leading us to investigate the link between aberrant glucose metabolism, T2DM, and AD in murine models. Here, we combined two techniques - glucose clamps and in vivo microdialysis - as a means to dynamically modulate blood glucose levels in awake, freely moving mice while measuring real-time changes in amyloid-beta (Abeta), glucose, and lactate within the hippocampal interstitial fluid (ISF). In a murine model of AD, induction of acute hyperglycemia in young animals increased ISF Abeta production and ISF lactate, which serves as a marker of neuronal activity. These effects were exacerbated in aged AD mice with marked Abeta plaque pathology. Inward rectifying, ATP-sensitive potassium (K(ATP)) channels mediated the response to elevated glucose levels, as pharmacological manipulation of K(ATP) channels in the hippocampus altered both ISF Abeta levels and neuronal activity. Taken together, these results suggest that K(ATP) channel activation mediates the response of hippocampal neurons to hyperglycemia by coupling metabolism with neuronal activity and ISF Abeta levels. |
